pGLO Lab Conclusion

pGLO Observations , Data Recording & Analysis

1.

Obtain your team plates.  Observe your set of  “+pGLO” plates under room light and with UV light.  Record numbers of colonies and color of colonies. Fill in the table below. Plate Number of Colonies Color of colonies under room light Color of colonies under   UV light - pGLO LB 0 carpet clear-ish tan light tan - pGLO LB/amp 0 carpet clear clear tan + pGLO LB/amp 75 musky tan color tan color + pGLO LB/amp/ara 250 musky tan color green

2.	What two new traits do your transformed bacteria have? Ampiciline resistance, and the ability to glow
3.	Estimate how many bacteria were in the 100 uL of bacteria that you spread on each plate. Explain your logic. About a thousand bacteria we in the 100 uL that I spread because we equally distributed 25 uL in all 4 dishes and then multiplying it by 10 (amounts of bacteria). 1000.
4.	What is the role of arabinose in the plates? The sugar that makes stuff glow.
5.	List and briefly explain three current uses for GFP (green fluorescent protein) in research or applied science. It can be used to observe host-parasite interactions so scientists can study different pathogens , also can create an image for pathogenic bacteria in which it gives a measurement of bacterial association. It is also an indicator.
6.	Give an example of another application of genetic engineering. Genetic Engineering has many applications including the production of human growth, insulin, vaccines and other types of drugs as well. To discover certain gene functions is the research for organisms that are genetically engineered.